News

Announcements

New Postdoc 🎉

Our laboratory is very pleased to welcome Agathe Depraz Depland, who started a postdoctoral position on February 1st, 2026.
She will focus on LC-HRMS coupling using innovative analytical conditions for the detailed characterization of next-generation protein- and nucleic acid-based therapeutics.
Welcome, Agathe!

Publication

doi
Investigating the total breakthrough behavior of oligonucleotides in ion-pairing chromatography and comprehensive HILIC Ă— IP-RPLC
In: Journal of Chromatography A, January 2026
By: Megane Aebischer, Yusraa Abdurahman, et al.

  • Demonstration of the Total Breakthrough behavior of oligonucleotides
  • Identification of key factors influencing breakthrough in HILIC Ă— IP-RPLC
  • Development of a comprehensive HILIC Ă— IP-RPLC method leveraging this behavior for oligonucleotide analysis.

Publication

doi
Systematic evaluation of phenyl stationary phases for oligonucleotide analysis without ion-pairing reagents
In: Journal of Chromatography A, November 2025
By: Athanasios Tsalmpouris, et al.

  • Alternative selectivity governed by Ď€-Ď€ stacking interactions
  • Absence of ion-pairing agents: No need to dedicate instruments as in IP-RPLC
  • MS friendly methods using regular volatile salts (ammonium acetate)

Feature

See Online
2D-LC Smart Calculator was featured on the front cover of the October issue of LCGC International

This tool, accessible on our website, aims to assist users, including either beginners or more experienced users seeking to fully optimize their analytical conditions and better understand the impact of parameters.
We hope this tool will encourage more users to adopt comprehensive LCĂ—LC, a powerful and underutilized analytical technique for resolving complex mixtures.

Publication

doi
A proof of concept study on the use of online two-dimensional SEC-UV-RPLC-MS method for the multi-attribute characterization of gene therapy products
In: Analytica Chimica Acta, October 2025
By: Megane Aebischer, et al.

  • 2D-LC (SEC-UV-RPLC-MS) as multi-attribute method for rAAV products characterization.
  • rAAV on-line denaturation between both dimensions.
  • Confirmation of 2D-LC's potential for gene therapy products characterization.

Publication

doi
Anion Exchange Chromatography to Determine mRNA Encapsulation in Lipid Nanoparticles
In: Analytical Chemistry, August 2025
By: Athanasios Tsalmpouris, Sofiane Mahjoubi, Jonathan Maurer, et al.

  • Separation of free vs. encapsulated mRNA under non-disruptive for LNP conditions
  • Mitigation of mRNA/LNP carryover using optimised wash protocols (high pH, high salt, surfactant)
  • Detection of additional species, as surface-bound and transmembrane mRNA species — providing structural insights beyond conventional methods

Awards

2025
Davy GUILLARME was the recipient of the Teaching award from the Faculty of Science of the University of Geneva, based on the students' vote
2025
Davy GUILLARME was the recipient of the Csaba Horvath Memorial award from the Hungarian Society for Separation Sciences and the Connecticut Separation Science Council
2025
Athanasios TSALMPOURIS won the Best poster 2nd price award at the HPLC2025 conference in Bruges, Belgium, June 2025 Poster title: Anion exchange chromatography to determine mRNA encapsulation in lipid nanoparticles
His contribution was selected among > 600 posters
2025
Athanasios TSALMPOURIS won the Best poster 1st price award in the category “biopharmaceuticals” at the HPLC2025 conference in Bruges, Belgium, June 2025 Poster title: Anion exchange chromatography to determine mRNA encapsulation in lipid nanoparticles