Equipments

The Phytochemistry and Bioactive Natural Products Research Unit is optimally equipped for metabolite profiling and annotation, isolation and structure elucidation of natural products.

METABOLITE PROFILING

Photo_Equipement_1_10.8.jpg

  • Q Exactive Focus, Orbitrap mass spectrometer (UHPLC-PDA-CAD-HR- MS/MS) (Thermo)
  • UHPLC-PDA-ELSD-Qda (Waters)
  • HPLC-PDA-ELSD reverse phase (3 instruments) (Agilent)
  • HPLC-PDA-ELSD normal phase (Agilent)

NATURAL PRODUCTS ISOLATION

Photo_Equipement_2_10.8.jpg

Small scale (mg of extracts/fractions)

  • Semi-preparative HPLC-UV-ELSD-MS (Shimadzu/Advion)
  • Semi-preparative Puriflash-UV-ELSD (Interchim)
  • Spot Prep HPLC-UV (Interchim)

Large scale (g of extracts/fractions)

  • Medium pressure LC Flash / MPLC-PDA-ELSD (Buchï)
  • HSCCC-Talto (260 mL)
  • HSCCC-Quattro (1000 mL)

All instruments are equipped with automated fractions collectors

Handling of extract/fractions/pure compounds at all scales

  • Accelerated Solvant Extraction Dionex
  • SpeedVac
  • Genevac
  • Lyophilisation
  • Rotavapor (Buchi)
  • Multivapor
  • SyncorePlus evaporator

STRUCTURAL IDENTIFICATION

Photo_Equipement_3_10.8.jpg

  • RMN 600 MHz (QCI 5mm Cryoprobe and a SampleJet automated sample changer) (Bruker)
  • Q Exactive Focus, Orbitrap mass spectrometer (UHPLC-PDA-CAD-HR- MS/MS) (Thermo)
  • UV and IR (Hach)
  • Optical rotation and ECD (Jasco)