Equipments
The Phytochemistry and Bioactive Natural Products Research Unit is optimally equipped for metabolite profiling and annotation, isolation and structure elucidation of natural products.
METABOLITE PROFILING
- Q Exactive Focus, Orbitrap mass spectrometer (UHPLC-PDA-CAD-HR- MS/MS) (Thermo)
- UHPLC-PDA-ELSD-Qda (Waters)
- HPLC-PDA-ELSD reverse phase (3 instruments) (Agilent)
- HPLC-PDA-ELSD normal phase (Agilent)
NATURAL PRODUCTS ISOLATION
Small scale (mg of extracts/fractions)
- Semi-preparative HPLC-UV-ELSD-MS (Shimadzu/Advion)
- Semi-preparative Puriflash-UV-ELSD (Interchim)
- Spot Prep HPLC-UV (Interchim)
Large scale (g of extracts/fractions)
- Medium pressure LC Flash / MPLC-PDA-ELSD (Buchï)
- HSCCC-Talto (260 mL)
- HSCCC-Quattro (1000 mL)
All instruments are equipped with automated fractions collectors
Handling of extract/fractions/pure compounds at all scales
- Accelerated Solvant Extraction Dionex
- SpeedVac
- Genevac
- Lyophilisation
- Rotavapor (Buchi)
- Multivapor
- SyncorePlus evaporator
STRUCTURAL IDENTIFICATION
- RMN 600 MHz (QCI 5mm Cryoprobe and a SampleJet automated sample changer) (Bruker)
- Q Exactive Focus, Orbitrap mass spectrometer (UHPLC-PDA-CAD-HR- MS/MS) (Thermo)
- UV and IR (Hach)
- Optical rotation and ECD (Jasco)