Mass spectrometry for biophysics

New concepts in mass spectrometry measurements and interpretation

Mass spectrometry of oligonucleotides and nucleic acids

Mass Spectrometry for Biophysics

Biophysical assays to test target structure and ligand binding stoichiometry, affinity, specificity and binding modes are part of the drug development pipeline. Mass spectrometry offers unique advantages as a biophysical method due to its ability to distinguish each stoichiometry present in a mixture. In addition, advanced mass spectrometry approaches (reactive probing, fragmentation techniques, ion mobility spectrometry, ion spectroscopy) provide more detailed information on the complexes.

Previous work: This has been a key topic of the Gabelica group for over 20 years, summarized in a comprehensive review of fundamentals of mass spectrometry and all its particularities when studying non-covalent nucleic acid structures. We also reviewed what has been learned thanks to mass spectrometry on nucleic acid structures, self-assemblies (e.g., duplexes or G-quadruplexes), and their complexes with ligands [1]. New projects are outlined below. TOC smaller.png

Key reference: Largy, E.; König, A.; Ghosh, A.; Ghosh, D.; Benabou, S.; Rosu, F.; Gabelica, V. “Mass Spectrometry of Nucleic Acid Noncovalent Complexes” Chem. Rev. (2022), 122(8), 7720-7839.

How drugs interfere with protein/G-quadruplex interactions

Conformational changes induced by ligand binding to their target