Setup for live cell fluorescent imaging microscopy. This imaging setup shown was used for the online measurements of biochemical signaling in living cells stimulated using different approaches. It consists of a monochromator and a Spectra-X 7 channels LED light source that allows the selection of a specific wavelength and the quick shift between several wavelengths, a high sensitivity sCMOS camera, an inverted microscope with objectives equipped for high resolution fluorescence imaging, a perfusion chamber, a manifold that allows the quick and swift introduction of 8 different buffers to cells under a specific field and a data acquisition and analysis software.


Live cell fluorescent imaging microscopy