Biomedical and Metabolomic Analysis
Innovation in Instrumentation and Analytical Strategies
New approaches in LC-MS for metabolomics
- Development of extended coverage single-run analyses
- Innovative LC-MS conditions for metabolomics and lipidomics
- Handling and analysis of volume-restricted samples
- High-throughput quantitative metabolomics
- CE-MS: development and evaluation of new interfaces
- Tools and libraries to use of effective electrophoretic mobility in compound identification
- Development and deployment of low-cost/open source CE instrumentation for falsified medicines
- Micro-format LC-MS setup for volume-restricted samples
- Solid phase micro-extractions: online SPE and μSPE
- Handling of atypical biological fluids: cerebrospinal fluid, cell cultures, seminal fluid, etc.
Metabolomics, Toxicology and Biomedical Analyses
Multi-platform metabolomics
- Targeted and untargeted metabolomics in CE- and LC-MS(/MS)
- Libraries and databases of standard compounds
- Multi-platform data merging methodologies
- Peak recognition methodologies and data annotation
- Retention time modelling
- Application of omics techniques in toxicity risk assessment
- Study design, metabolomic analyses and biological interpretation
- Serum, plasma, urine, seminal fluid analysis (>200 endogenous steroids)
- Seminal fluid quality for male infertility
- Endocrine disruption (H295R OECD model)
- Steroidogenesis alteration
- Deep steroid recognition and annotation
- Steroidome alterations
- Alterations of polar metabolome
- Cellular models (2D and 3D)
- Polar compound analysis
Data Analysis and Interpretation
Multivariate analysis
- Dimensionality reduction
- Knowledge extraction
- Machine learning
- Multiblock analysis
- Multiway analysis
- Megavariate analysis of variance
- Factor screening
- Response surface for process optimization
- Accuracy and uncertainty profiles